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AFP (Alpha-Fetoprotein)

AFP — tumor marker used in diagnosis and follow-up of hepatocellular carcinoma and germ cell tumors.

Alpha-fetoprotein blood test used in the diagnosis, follow-up, and recurrence monitoring of liver cancer (hepatocellular carcinoma) and germ cell tumors.

Indication

  • Diagnosis and follow-up of hepatocellular carcinoma (HCC)
  • Liver cancer screening support in cirrhosis patients (combined with imaging)
  • Diagnosis/follow-up of germ cell testicular and ovarian tumors
  • Monitoring treatment response and assessing recurrence
  • Fetal anomaly screening in pregnancy (component of triple/quadruple screening)
  • Periodic evaluation in chronic hepatitis B/C follow-up

Preparation

  • No special fasting required
  • Pregnancy status must be reported (reference range varies)
  • Disclosure of any history of chronic liver disease
  • Bringing previous AFP and imaging results

How it's performed

  1. A venous blood sample is drawn from the arm
  2. Measurement is performed on serum using immunological methods
  3. Result is reported in ng/mL
  4. In HCC follow-up, use together with liver ultrasound every 6 months is recommended

Post-procedure

  • Regular measurement during treatment
  • If values rise, abdominal imaging (US, MRI, CT) is performed
  • Trend monitoring is more valuable than a single measurement
  • Results are interpreted by a physician

Risks

  • Minor bruising or tenderness related to blood draw
  • False elevation in conditions such as pregnancy, hepatitis, and cirrhosis
  • Normal values in early-stage HCC (false negative)
  • Cannot establish diagnosis on its own; evaluated together with imaging

FAQ

Does an elevated AFP always mean liver cancer?

No. It can also rise in hepatitis, cirrhosis, pregnancy, and certain benign conditions. It is evaluated together with imaging.

I have cirrhosis — how often should AFP be checked?

Most guidelines recommend AFP and liver ultrasound every 6 months in cirrhosis patients. Follow your physician's recommendation.

Why is AFP measured during pregnancy?

It is part of second-trimester screening for fetal neural tube defects and chromosomal anomalies; it does not establish a diagnosis on its own.